FIG. 5.

Flow cytometric analysis of TF antigen expression on the surfaces of monocytes or EC after coculture. Monolayers of EC were incubated for 24 h in culture medium alone, with 5 ng of rIL-1/ml, or with the indicated bacteria. These monolayers were subsequently washed and cultured for 5 h in the presence of monocytes. Then, EC and EC-adherent monocytes were analyzed separately for membrane expression of TF molecules as described in Materials and Methods. Open profiles represent the cells before coculture. Closed profiles represent the cells after 5 h of coculture. Data from one representative experiment are shown.