Fig. 7. CDKN1A is involved in a trehalose-induced increase in the expression of wound healing-related molecules.

After transfection with control or CDKN1A siRNA, human dermal fibroblasts were treated with trehalose (100 mg/ml) or vehicle control (PBS) for 48 h. CDKN1A, DPT, ANGPT2, VEGF, EREG, and FGF2 expressions were assessed by qPCR. Data were shown as relative expression to the control siRNA- and vehicle-treated fibroblasts. Data were expressed as means ± SD and are representative of two independent experiments with similar results. *P < 0.05, **P < 0.01, and ****P < 0.0001 versus the relevant control group, and ##P < 0.01, ###P < 0.001, and ####P < 0.0001 versus the control siRNA-treated group of vehicle-treated fibroblasts by two-way ANOVA.