Skip to main content
. 2023 Jan 6;14:108. doi: 10.1038/s41467-022-35732-1

Fig. 6. Specific ablation of Mfn1 in skeletal muscle promotes TLR9-dependent NFκB-dependent inflammation, muscle atrophy, reduced physical performance, and enhanced IL6 response to exercise.

Fig. 6

a Mfn1 and Tubulin representative immunoblot in total homogenates of quadriceps muscles of LoxP (n = 4 mice) and SkM-Mfn1KO (n = 4 mice) male and female mice. b TEM images from cross-sectional sections of quadriceps muscles of LoxP and SkM-Mfn1KO male mice (n = 4 mice) and quantification of parameters representing mitochondrial morphology (Scale bar, 500 nm). c NFκB target and type I IFN response gene expression in quadriceps muscles of LoxP (n = 8 mice) and SkM-Mfn1KO mice (n = 14 mice). d NFκB target gene expression in quadriceps muscles of endotoxin-free water- or ODN2088-treated (100 µg/mice, necropsy 48 h after injection) LoxP (n = 4 mice) or SkM-Mfn1KO male mice (n = 6 mice). e Representative images of hematoxylin/eosin staining of cross-sectional sections of gastrocnemius muscles (Scale bar, 100 µm) and quantification of the CSA (n = 3 mice; 4 areas per mice; 30 fibers per area). f CK activity in plasma samples (n = 16 mice). g Fgf21 mRNA levels in quadriceps muscles of LoxP (n = 6 mice) and SkM-Mfn1KO male mice (n = 5 mice). h Plasma FGF21 levels in LoxP (n = 7 mice) and SkM-Mfn1KO male mice (n = 6 mice). i Distance run on the treadmill test and the difference in the distance run between day 2 and day 1 in LoxP (n = 12 mice) and SkM-Mfn1KO male mice (n = 11 mice). j Il6 expression levels in quadriceps muscles and k plasma IL6 levels of LoxP and SkM-Mfn1KO male mice at resting conditions or at different time-points after the treadmill test (n = 14 mice). b, e–k Two-sided Students’ T test, c Two-sided Students’ T-test per gene, and d two-way ANOVA test and post hoc t tests. Data are expressed as mean ± SEM. *p vs. LoxP <0.05. a–k Source data is provided in the Source Data File.