Table 3.
Primer sequences used in this study.
| Gene | Primer | Sequence (5′ to 3′) | GenBank ID | Size, bp |
|---|---|---|---|---|
| GAPDH | Forward Reverse |
AAGGAGTAAGAGCCCCTGGA TCTGGGATGGAAACTGGAA |
NM_001206359.1 | 140 |
| CEBPα | Forward Reverse |
AACAACTGAGCCGCGAACTGGA CTTGAGATCTGGAGACCCGAAACC |
XM_003127015.4 | 170 |
| PPARγ | Forward Reverse |
AAGACGGGGTCCTCATCTCC CGCCAGGTCGCTGTCATCT |
NM_214379.1 | 149 |
| SREBP1 | Forward Reverse |
AAGCGGACGGCTCACAATG CGCAAGACGGCGGATTTAT |
NM_214157.1 | 122 |
| ACCα | Forward Reverse |
TTCCAGGCACAGTCCTTAGG TCATCCAACACGAGCTCAGT |
NM_001114269.1 | 161 |
| FABP4 | Forward Reverse |
TGGAAACTTGTCTCCAGTG GGTACTTTCTGATCTAATGGTG |
NM_001002817.1 | 147 |
| ACAA1 | Forward Reverse |
CGAGCTTCTCTCTGCAGTCAT TGGGATGTCACTCAGAAACTGG |
XM_003132103.4 | 148 |
| FADS2 | Forward Reverse |
GCTGGATTCCAACCCTCATG AGCCTGGGCCTGAGAGGTA |
NM_001171750.1 | 56 |
| SCD | Forward Reverse |
GCCACCTTTCTTCGTTACG CCTCACCCACAGCTCCCAAT |
NM_213781.1 | 142 |
GAPDH = glyceraldehyde-3-phosphate dehydrogenase; CEBPα = CCAAT enhancer-binding protein α; PPARγ = peroxisome proliferator-activated receptor γ; SREBP1 = sterol regulatory element binding protein 1; ACCα = acetyl CoA carboxylase α; FABP4 = fatty acid-binding protein 4; ACAA1 = acetyl-CoA acyltransferase 1; FADS2 = fatty acid desaturase 2; SCD = stearoyl Coenzyme A desaturase.