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. Author manuscript; available in PMC: 2023 Jan 7.
Published in final edited form as: Xenobiotica. 2019 Nov 25;50(7):805–814. doi: 10.1080/00498254.2019.1694197

Table 5.

Michaelis–Menten kinetics for formation of AC-1 metabolite in rat microsomes over a range of AC concentrations (3.125–350 μM) at 37 °C, 120 min, 2 mg/mL protein, 5 mM NADPH, 100 mM K2HPO4 pH 7.4.

PREPARATION Km (μM)±SE Vmax (pmol/min/mg)±SE

#1 – Lot# 1410271 70.6 ± 12.6 4.62 ± 0.24
#2 – Lot# 1610290 76.0 ± 14.6 4.70 ± 0.27
#3 – Lot# 1310214 64.0 ± 8.3 2.98 ± 0.11
Mean of Individual Rates 70.2 ± 6.0 4.10 ± 0.97
Mean Rate Across Preparations 71.0 ± 27.7 4.10 ± 0.47