. 2023 Jan 6;13:308. doi: 10.1038/s41598-022-27236-1

Table 1.

Description of the variables.

Variable	Description	Data type	Importance
Field approach
Infection frequency (IF)	Represents the natural infection of mosquitos in natural conditions $I F = \frac{t i m e s p o s i t i v e}{t i m e s t e s t e d b y s p e c i e s} \times (1 + l o g 10)$	Discrete	This variable indicates the receptivity of mosquitos to natural infection
Standardized minimum infection rate (SMIR)	The MIR is the ratio of the number of positive pools to the total number of mosquitoes in the sample. It is the minimum infection proportion, and it assumes that only one infected individual is present in a positive pool³⁸	Continuous	Represents the magnitude of the natural infection. If the virus is detected in dissected mosquitoes. However, it does not necessary represents an infection in the salivary glands
Infection risk (IR)	Represents the infection potential based on IF and SMIR $I R = I F \times S M I R$	Continuous	It combines the frequency and magnitude of a mosquito species to become naturally infected with the virus in the field
Experimental approach
Transmission Frequency (TF)	Represents the presence of the virus in the mosquito saliva after an experimental infection $T F = \frac{t i m e s p o s i t i v e}{t i m e s t e s t e d b y s p e c i e s} \times (1 + l o g 10)$	Discrete	It shows the ability of each mosquito species to excrete the viruses every time infected. But it does not represent a magnitude of potential
Standardized transmission efficiency (STE)	TE, showing the proportion of individuals with infectious saliva among all individuals infected¹⁵	Continuous	The variable indicates the capacity of a mosquito to transmit the virus. But does not reflect the viral titer and the infectivity of viral particles presents in the saliva
Transmission risk (TR)	Describes the transmission potential based on the product of TF and the STE $T R = T F \times S T E$	Continuous	It combines de frequency and magnitude of a mosquito species to transmit the virus in experimental conditions
Infection-transmission risk (ITR)	Represent the total risk based on the field and laboratory data	Continuous	It considers the transmission risk in experimental conditions and the natural infection risk