Table 1:

Example six-week UCMS schedule

Sunday	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday
Tail cramp (1 min) Heat stimulation (10 min)	Restraint (2 hrs) Loud music (3 hrs) Exposure to empty water bottle (overnight)	Wet bedding (3 hrs) 45° cage tilt (7 hrs)	Shake on rocker (1 hr) Cold exposure (30 mn) Spray with water (5 min)	No bedding (4 hrs) Heat stimulation (10 min) Restraint (2 hrs)	Loud music (3 hrs) Crowded space (1 hr)	Spray with water (5 min) Tail cramp (1 min) 45° cage tilt (overnight)

Mice were exposed to 2-3 stressors each day for a period of six consecutive weeks. Of note, to prevent animals from habituating to the daily stressors, these stressors were performed at different times throughout the day (i.e., in the morning, afternoon, evening, and overnight).