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. 2022 Dec 21;102(3):102441. doi: 10.1016/j.psj.2022.102441

Figure 4.

Figure 4

Effect of a LPD supplemented with isoleucine (Ile) on the AMPK/mTOR and Leptin/JAK2/STAT3 signaling pathways in the liver of broilers. (A) Western blot analysis of proteins associated with the AMPK/mTOR signaling pathway. (B) Relative expression level of proteins associated with the AMPK/mTOR signaling pathway. (C) LEPR gene expression level. (D) Western blot analysis of proteins associated with the JAK2/STAT3 signaling pathway. (E) Relative expression level of proteins associated with the JAK2/STAT3 signaling pathway. AMPK, 5′ adenosine monophosphate-activated protein kinase; mTOR, mechanistic target of rapamycin; p70S6K, ribosomal protein 70 S6 kinase; SREBP1, sterol regulatory element-binding protein 1; LEPR, leptin receptor; JAK2, janus kinase 2; STAT3, signal transducer and activator of transcription 3; CON, control diet; LPD, low-protein diet; LPD-LI, low-protein diet + 0.13% Ile; LPD-HI, low-protein diet + 0.26% Ile. a,b,cBars with no common superscript differ significantly (P < 0.05).