Table 2.
Primers used for real time-PCR.
| Gene1 | Primer sequence (5’ to 3’) | Product size (bp) | GenBank no. |
|---|---|---|---|
| MyHC-I | F: AACGCCGCAACAACCT R: TTCTTCTTCATCCGCTCC |
330 | U85023.1 |
| MyHC-IIa | F: CCACCAATCCATACGACT R: CTGGCTCTGCTTGCTCT |
216 | NM 204228.1 |
| MyHC-IIb | F: TGTGAGTCAAGGCGAGAT R: CCAGAGCACCTACAGCAT |
347 | NM_001013396.1 |
| LXRα | F: GTGCAGAGAGTGACGAGCTT R: AGAGGTTTACGTGCGTGGAG |
175 | NM_204542 |
| SREBP-1 | F: TCACCGCTTCTTCGTGGAC R: CTGAAGGTACTCCAACGCATC |
220 | AY029224 |
| ACC | F: AATGGCAGCTTTGGAGGTGT R: TTCTGTTTGGGTGGGAGGTG |
137 | NM_205505.2 |
| FAS | F: AACTCTCTGCCATCTCCCGA R: TAGCCTGGTAGCCAGTTCGT |
165 | NM_205155.4 |
| PPARα | F: AATCATAAAGGAGTTTAAGTGACCG R: GCTGGTGAAAGGGTGTCTGT |
264 | NM_001001464.1 |
| CPT-1 | F: GGCTCTGGCAGGAGCTACA R: AGTAGGTCAGGACACGCTCA |
108 | XM_040700878.1 |
| ACOX1 | F: ATGTCACGTTCACCCCATCC R: AGGTAGGAGACCATGCCAGT |
133 | NM_001006205 |
| PPARγ | F: CAGTGGATCTGTCTGCGATG R: CTTTGGCAATCCTGGAGCTTG |
172 | NM_001001460 |
| C/EBPα | F: ATGGAGCAAGCCAACTTCTAC R: GCCAGGAACTCGTCGTTGAA |
230 | NM_001031459 |
| KI67 | F: AGGATGGAAGCAAGTCACCTGGAT R: CTTCTGAACGGGGACTGGAATCTT |
129 | XM_004020367 |
| TOP2A | F: TAAGCCCACTAGCACGGTTG R: CCAATCCCTTCTGCCCCATT |
142 | NM_204791.3 |
| TPX2 | F: TGGAGGGTGGGCCAATC R: TTGGCTGTGTGAGTTCCTTCAC |
57 | NM_204437.1 |
| LEPR | F: TCCCCCAGTGACCCGACCTCT R: CGCCTCATTTCCCAGCTCCCA |
201 | NM_204323 |
| GAPDH | F: GGGCACGCCATCACTATCTT R: TAACACGCTTAGCACCACCC |
148 | NM_204305.1 |
1
MyHC, myosin heavy chain; LXRα, liver X receptors-α; SREBP1, sterol regulatory element binding protein 1; ACC, acetyl-CoA carboxylase; FAS, fatty acid synthase; PPARα, peroxisome proliferator activated receptor-α; CPT-1, carnitine palmitoyl-transferase 1; ACOX1, acyl-CoA oxidase 1; KI67, ki-67; TOP2A, topoisomerase II alpha; TPX2, thioredoxin-dependent peroxidase 2; PPARγ, peroxisome proliferator-activated receptor-γ; C/EBPα, CCAAT/enhancer binding protein-α; LEPR, leptin receptor; GAPGH, glyceraldehyde-3-phosphate dehydrogenase.