Table 1.
Pyrogallol auto-oxidation assay on SDBC samples
| Time |
−SDBC |
+SDBC |
Antioxidant effecta |
|---|---|---|---|
| Minutes | Δ absorbance (AU)b | (%) | |
| 0 | 0 | 0 | 0 |
| 1 | 0.950 ± 0.131 | 0.139 ± 0.027 | 85.40 |
| 2 | 0.923 ± 0.132 | 0.173 ± 0.008 | 81.30 |
| 3 | 0.813 ± 0.055 | 0.157 ± 0.014 | 80.66 |
| 4 | 0.663 ± 0.032 | 0.133 ± 0.023 | 79.90 |
| 5 | 0.563 ± 0.055 | 0.107 ± 0.020 | 80.95 |
The rates of pyrogallol auto-oxidation were used as an indication of the ROS superoxide ion (O2−) scavenging activity of the SDBC and therefore of its Cu-only SOD subunit. The data show that in presence of the SDBC, the auto-oxidation at 1 min decreased by ∼85% compared to controls. This antioxidant activity was monitored for 5 min showing a similar effect.
a
SDBC antioxidant effect expressed as rate (%) of pyrogallol protection with respect to the control (−SDBC); each value is the mean of three independent measurements.
b
Difference of absorbance between measurements performed at a distance of 1 minute.