Fig. 1.

Proteomic Profiling of Mouse Oocyte Maturation.A, schematic overview of the workflow for proteome profiling of mouse oocytes isolated at GV, GVBD, and MII stages. B, Venn diagram of protein numbers identified in different studies. C, principal component analysis of protein expression patterns of three different stages. D, diagram of proteins distribution among subcellular compartments. E–K, unsupervised c-means fuzzy clustering identified seven distinct profiles of proteins expression. The x-axis represents three developmental stages. The y-axis represents normalized intensity value and log2-transformed. Gene ontology analysis is shown on the right panel of each cluster. L, percentage of the protein number in each cluster. M, the yellow-colored bars indicate the percentage of maternal proteins in each cluster. N, pie chart shows the number of maternal proteins in each cluster. The right line chart represents the expression patterns of partial maternal proteins.