Fig. 6.

CRLs modulate oocyte maturation by Controlling the Degradation of Actin-Related Proteins.A, pie plot showing the Cullin-RING ubiquitin ligases (CRLs) and other ligases identified in the proteome. B, schematic diagram showing that four complexes of CRLs are inhibited by MLN4924. C, schematic presentation of the experimental design to investigate the effect of CRLs inhibition on oocyte maturation. D, bright-field images of oocytes treated with dimethyl sulfoxide (DMSO) and MLN4924. The scale bar represents 100 μm. E, percentage of four meiotic stages (GV, GVBD, MI, and MII) in oocytes treated with DMSO and MLN4924. F, overview of a proteomic method for identifying differential proteins. G, Venn diagram showing the exclusive or shared upregulated proteins in oocytes treated with DMSO and MLN4924. H, GO enrichment analysis. The bar plot depicts the top five enriched biological processes of 210 common proteins shown in Fig. 6G. I, heatmap showing the differentially expressed proteins related to actin binding. J and K, heatmap showing the expression of partial proteins related to actin organization. Data are expressed as the mean ± SD from three independent experiments in which at least 100 oocytes were analyzed for each group. For statistical analysis, a two-tailed Student’s t test was used in all panels, compared with GV or control. ∗∗∗p < 0.001.