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. 2022 Dec 19;26(1):105837. doi: 10.1016/j.isci.2022.105837

Figure 1.

Figure 1

Characterization of the Mz317 glia in the third antennal segment

(A) Double labeling of OR neurons expressing the ORNs (in magenta) and Mz317 glia (in green) in a 40-μm-thick cryosection observed by confocal microscopy. Above the image of ORNs and Mz317 in the third antennal segment. The drawing on the right represents the third antennal segment, and a magnification of the region in the square is presented below. The Mz317 glia closely surround the soma of the ORNs, forming a network structure.

(B) Above, schematic design of the GRASP experiment expressing the spGFP1-10 semimarker in Mz317 glia and the spGFP11 semimarker in ORNs. GFP labeling is only observed when both semimarkers bind. Bottom, 14-μm antennal cryosections with GFP labeling (in green) in the third antennal segment of experimental individuals but not in controls expressing only one of the semimarkers. In all photos, the scale bar indicates 40 μm.