Figure 5.

Effects of RNAi silencing of several candidate genes in MZ317 cells on olfactory behavior in response to ethyl acetate (10^−1.5)

RNAi lines were generated by the TRiP program, which also produced control lines for chromosome 2 and chromosome 3. We used 2 UAS-RNAi stocks for each gene except for Eaat2. In all cases, we compared the experimental flies, Mz317-Gal4/UAS-RNAi-GeneX, against the corresponding controls, Mz317-Gal4/UAS-36304 and MZ317-Gal4/UAS-36303, for chromosomes 2 and 3, respectively. The mean ± SEM values are presented. N = 20 for each fly type. T test values are from left to right; for chromosome 2, t-Irk2₃₈ = 0.107, n.s.; t-Eaat2₃₈ = 2.226∗; t-vGLUT₃₈ = −4.417∗∗∗; for chromosome 3, t-shakB-HM₃₈ = −6.491∗∗∗; t-shakB-JF₃₈ = −6.912∗∗∗; t-vGLUT₃₈ = −5.159∗∗∗; t-Irk1-HM₃₈ = −0.194, n.s.; t-Irk1-JF₃₈ = −1.122, n.s.; t-Irk2 ₃₈ = −1.172, n.s. Significant differences of each fly type compared with the control flies are indicated in the figure (∗∗∗ = p < 0.001; ∗∗ = p < 0.01; ∗ = p < 0.05).