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. 2022 Dec 19;26(1):105837. doi: 10.1016/j.isci.2022.105837

Figure 7.

Figure 7

The absence of Orco in orco−/orco− mutants does not affect the structure of antennal Mz317 glia but eliminates the response of Mz317 antennal glial cells

(A) Detail of the immunostaining of 14-μm antennal cryosections of several fly genotypes. Green (GFP) immunostaining labeling of Mz317 glia and magenta labeling of the Orco coreceptor in Mz317-Gal4/UAS-mCD8:GFP flies were used as positive controls, Mz317-Gal4/UAS-mCD8:GFP; orco-/orco-experimental flies and UAS-mcD8:GFP as negative control flies. The scale bar indicates 40 μm. The same Mz317 glial structure appears in the positive control and the orco−/orco− mutants.

(B) Left, representative fluorescence response of the Mz317 glial cells of an orco−/orco− mutant fly submitted to increasing odorant concentrations. Right, dose-response curve of the maximal response (mean ± SEM of 20 antennae) at the times when the response of Mz317 glial cells was observed. No significant response was observed among different odorant concentrations, and the difference from the 0 value corresponds to signal noise.