REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-GFP	Invitrogen	Cat#A6455; RRID: AB_221570
Alexa 488 polyclonal anti-rabbit	Invitrogen	Cat# A11008; RRID: AB_143165
Mouse monoclonal anti-nc82	Developmental Studies Hybridoma	Cat# Supernatant 1 ml; RRID: AB_2314866
Cy3 polyclonal anti-mouse	Jackson Immunoresearch Laboratories	Cat# 715-165-151; RRID: AB_2315777
Mouse monoclonal anti-GFP	Invitrogen	Cat#A11120; RRID: AB_221568
Rabbit polyclonal anti-ORCO	Larsson et al.88	N/A
Alexa 488 polyclonal anti-mouse	Invitrogen	Cat#A21131; RRID: AB_2535771
Cy3 polyclonal anti-rabbit	Jackson Immunoresearch Laboratories	Cat#111-165-003; RRID: AB_2338000
Chemicals, peptides, and recombinant proteins
Ethyl acetate	Sigma-Aldrich	Cat# 1.00863.0500; CAS: 141-78-6
3-octanol	Sigma-Aldrich	Cat# 218405-50G; CAS: 589-98-0
Methylsalicylate	Fluka	Cat# 76631; CAS: 119-36-8
Paraffin oil	Merck	Cat# 1.07174.2500; CAS: 8042-47-5
Experimental models: Organisms/strains
D. melanogaster: Canton-S	Bloomington Drosophila Stock Center	BDSC:64349; FlyBase: FBst0064349
D. melanogaster: Mz317-Gal4: w; Mz317-Gal4/CyO	Ito et al.89	N/A
D. melanogaster: orco-lexA: w; Bl/CyO; orco-lexA/TM6B	Lai and Lee90	N/A
D. melanogaster: repo-Gal80: w; repo-Gal80 (N18)/CyO	Awasaki et al.91	N/A
D. melanogaster: elav-Gal80: w; elav-Gal80/CyO; rotund-Gal4, UAS-GFPnls/TM6	Casas-Tintó et al.92	N/A
D. melanogaster: Gal80ts: w[∗]; P{w[+mC] = tubP-GAL80[ts]}2/TM2	Bloomington Drosophila Stock Center	BDSC:7017; FlyBase: FBst0007017
D. melanogaster: UAS-DTI: w[∗]; P{w[+mC] = UAS-Cbeta∖DT.I}18/CyO	Bloomington Drosophila Stock Center	BDSC:25039; FlyBase: FBst0025039
D. melanogaster: UAS-ChR2XXL: y[1]w[1118]; PBac{y[+mDint2] w[+mC] = UAS-ChR2.XXL}VK00018	Bloomington Drosophila Stock Center	BDSC:58374; FlyBase: FBst0058374
D. melanogaster: UAS-eNpHR: y[1] w[∗]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC] = 20XUAS-eNpHR3.0.YFP}attP2/TM6C, Sb[1] Tb[1]	Bloomington Drosophila Stock Center	BDSC:36368; FlyBase: FBst0036368
D. melanogaster: RNAi of dEaat2: y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS01998}attP40/CyO	Bloomington Drosophila Stock Center	BDSC:40832; FlyBase: FBst0040832
D. melanogaster: RNAi of shakB V20: y[1] sc[∗] v[1] sev[21]; P{y[+t7.7]v[+t1.8] = TRiP.HMC04895}attP2	Bloomington Drosophila Stock Center	BDSC:57706; FlyBase: FBst0057706
D. melanogaster: RNAi of shakB V10: y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02604}attP2	Bloomington Drosophila Stock Center	BDSC: 27292; FlyBase: FBst0027292
D. melanogaster: RNAi of VGlut chr 2: y[1] sc[∗] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS02175}attP40	Bloomington Drosophila Stock Center	BDSC: 40927; FlyBase: FBst0040927
D. melanogaster: RNAi of VGlut chr 3: y[1] sc[∗] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS02011}attP2	Bloomington Drosophila Stock Center	BDSC: 40845; FlyBase: FBst0040845
D. melanogaster: RNAi of Irk1 v20: y[1] sc[∗] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS02480}attP2	Bloomington Drosophila Stock Center	BDSC: 42644; FlyBase: FBst0042644
D. melanogaster: RNAi of UAS-Irk1 v10: y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF01841}attP2	Bloomington Drosophila Stock Center	BDSC: 25823; FlyBase: FBst0025823
D. melanogaster: RNAi of Irk2 chr 2: y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ22463}attP40	Bloomington Drosophila Stock Center	BDSC: 58333; FlyBase: FBst0058333
D. melanogaster: RNAi of Irk2 chr 3: y[1] sc[∗] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS02379}attP2	Bloomington Drosophila Stock Center	BDSC: 41981; FlyBase: FBst0041981
D. melanogaster: Control TRiP chr 2: y[1] v[1]; P{y[+t7.7] = CaryP}attP40	Bloomington Drosophila Stock Center	BDSC: 36304; FlyBase: FBst0036304
D. melanogaster: Control TRiP chr 3: y[1] v[1]; P{y[+t7.7] = CaryP}attP2	Bloomington Drosophila Stock Center	BDSC: 36303; FlyBase: FBst0036303
D. melanogaster: UAS-Dicer: P{w[+mC] = UAS-Dcr-2.D}1, w[1118]; betaTub60D[Pin-1]/CyO	Bloomington Drosophila Stock Center	BDSC: 24644; FlyBase: FBst0024644
D. melanogaster: UAS-GCaMP6f: w[1118]; P{y[+t7.7] w[+mC] = 20XUAS-IVS-GCaMP6f}attP40	Bloomington Drosophila Stock Center	BDSC: 42747; FlyBase: FBst0042747
D. melanogaster: lexAOp-GCaMP6f: w[1118]; P{y[+t7.7] w[+mC] = 13XLexAop2-IVS-GCaMP6f-p10}su(Hw)attP5	Bloomington Drosophila Stock Center	BDSC: 44277; FlyBase: FBst0044277
D. melanogaster: UAS-mCD8::GFP chr2: y[1] w[∗]; P{w[+mC] = UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2	Bloomington Drosophila Stock Center	BDSC: 5137; FlyBase: FBst0005137
D. melanogaster: UAS-mCD8::GFP chr3: y[1] w[∗]; betaTub60D[Pin-Yt]/CyO; P{w[+mC] = UAS-mCD8::GFP.L}LL6	Bloomington Drosophila Stock Center	BDSC: 5130; FlyBase: FBst0005130
D. melanogaster: orco-RFP: w[∗]; P{w[+mC] = UAS-mCD8::GFP.L}LL5/CyO; P{w[+mW.hs] = Orco-RFP.K}10D	Bloomington Drosophila Stock Center	BDSC: 63045; FlyBase: FBst0063045
D. melanogaster: UAS-spGFP1-10, lexAOp-spGFP11: w[∗]; P{y[+t7.7] w[+mC] = CoinFLP-LexA::GAD.GAL4}attP40 P{w[+mC] = lexAop-rCD2.RFP}2; P{w[+mC] = UAS-CD4-spGFP1-10}3, P{w[+mC] = lexAop-CD4-spGFP11}3/TM6C, Sb[1]	Bloomington Drosophila Stock Center	BDSC: 58755; FlyBase: FBst0058755
Software and algorithms
ImageJ	Schneider et al.93	https://imagej.nih.gov/ij/