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. 2022 Dec 1;64(1):100317. doi: 10.1016/j.jlr.2022.100317

Fig. 7.

Fig. 7

STGD3 is deficient in very long chain saturated FA synthesis. A: Uptake of 24:0 after 72 h in HEK293 cells overexpressing WT, STGD3, and control cells (GFP and UT) supplemented with 24:0 SFA. B: Total 26:0 in the cells supplemented with 24:0 SFA. C: VLC-SFA levels in cells described in A. VLC-SFAs in STGD3-expressing cells were not significantly different from GFP control cells and were therefore not normalized to WT ELOVL4 and W246G ELOVL4 protein expression. D: WT and STGD3 ELOVL4 protein expression after 24:0 supplementation. Cell lysates were collected after 72 h for Western blot analysis with beta-tubulin as loading control. Results are the mean ± SD (n = 3). Statistical significance was assessed for A, B, and C by ANOVA with Tukey’s post hoc test. ∗∗∗∗P < 0.0001, ns not significant in comparison with WT. ELOVL, elongation of very long-chain fatty acid; VLC-SFA, very long chain-saturated FA; STGD3, Autosomal Stargardt-like Macular Dystrophy; UT, untransduced.