Figure 4.

Glutaraldehyde cross-linking and heparinization of composite sponge scaffolds

(A) CADMS's and ADM's H&E staining, Masson's trichromatic and toluidine blue staining.

(B) SEM images before and after glutaraldehyde cross-linking.

(C) Fourier infrared spectra of glutaraldehyde before and after cross-linking.

(D) Tensile stress curves.

(E) Elastic modulus.

(F) Relative cell viability before and after glutaraldehyde cross-linking.

(G) Toluidine blue staining after heparinized modification.

(H) Determined absorbance of toluidine blue at OD⁶⁰⁰.

(I) Relative cell viability before and after heparin sodium modification.

(J) Changes of CADMS-G hygroscopicity before and after heparinization.

(K) Fourier infrared spectroscopy before and after heparinization.