Figure 3.

Exercise inhibited bone resorption and protected bone by reducing adipsin and Spp1. Quantitative real-time PCR analyses and protein expression level of tibia BM. (A) Bone remodeling related gene expression quantitative analysis of bone marrow. The bone marrow was isolated from the male C57/B6 mice (N = 9) that were induced for 6 weeks of high-fat diet and intervened in twelve weeks of exercise. * p < 0.05, ** p < 0.01, *** p < 0.001 for obesity group (N = 9) vs exercise group (N = 7). (B,C) Western blotting and quantification (N = 6) in tibia bone marrow from obesity group and exercise group. * p < 0.05, for obesity group (N = 6) vs exercise group (N = 6). * p < 0.05, ** p < 0.01 2-sided Student t tests were used for statistical analyses. Data are presented as mean ± SD. BMAT, bone marrow adipose tissue; PCR, polymerase chain reaction.