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. 2022 Dec 30;15(1):179. doi: 10.3390/polym15010179

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) HABA/Avidin assay for the determination of BT bound to the F127. Schematic insert of HABA/Avidin behavior in the presence of biotin. (B) Kinetic profile of fluorescamine with spermine addition. Insert calibration curve obtained to determine the percentage of SN bound to the F127. (C) UV-Vis electronic absorption spectra and curve obtained for the determination of the percentage of AF bound to F127 (30.0 °C, λ_max = 290 nm). The black arrow on the side indicates the direction of the fluorescence signal with the titrations.