Figure 3.

(a) Histological evaluation of the skin morphology (I. Hematoxylin), skin permeability effects (II. Lucifer Yellow), skin barrier effects (III. Filaggrin) and (b) area ratio of stratum corneum of healthy and atopic dermatitis full-thickness skin models after 12 days of cultivation to air surface (airlift). Atopic dermatitis skin models were stimulated with T_H2 cytokines (50 ng/mL IL-4, 50 ng/mL IL-13 and 25 ng/mL IL-31) at day 0, 2, 5, 7 and 9 (m2, m3). Healthy skin model m4 and atopic dermatitis skin model m3 were stimulated with 10 µM histamine at day 9. Healthy skin models were cultivated under normal medium conditions (m1, m4). (a) Skin models for evaluation of skin morphology were stained with haematoxylin/eosin (I), for evaluation of skin permeability with Lucifer Yellow dye (II) and evaluation of skin barrier effects with filaggrin (III). Scale bar: 50 µm. (b) The ratio of the stratum corneum in whole skin models was calculated as the area of the stratum corneum compared to the area of the whole skin model and is given as mean ± SEM in [%] using the image processing program ImageJ (Scale bar: 100 µm). Histological analyses were conducted on three skin models from two independent experiments with four images per skin model. Asterisks [*] indicate significant deviations from the untreated control (*** p < 0.001).