FIG. 5.

(A) Window net analysis of the C-terminal antibody-binding region. The results show the IgG reactivities of hyperimmune sera from mice immunized with electrophoretically purified mp58 against all overlapping tetra-, penta-, hexa-, hepta-, octa-, nona-, and decapeptides spanning the C terminus of mp58. The results represent anti-IgG reactivity (measured as optical density at 490 nm [OD=490]; average values of two experiments using hyperimmune sera from mice immunized with electrophoretically purified mp58). (B) Results of the replacement net experiment. The epitopic region HTHADGEVH identified in previous analysis was the basis for synthesis of single-residue replacement analogs. Alanine was substituted for each residue one at a time along the C-terminal reactive peptide, and the alanine in the original sequence was replaced by glycine. The results represent the percentage of reactivity (anti-IgG; average of two experiments using hyperimmune sera from mice immunized with electrophoretically purified mp58) retained by peptides with the indicated residue replaced compared to the reactivity observed with the native (unsubstituted) peptide.