Table 8.
GC-MS/MS method conditions.
| Parameter | GC-MS/MS |
|---|---|
| Injection mode | Hot-splitless; MMI injection mode |
| Injection volume | 2 μL |
| Inlet temperature | 280 °C |
| Carrier gas | He, constant flow 1.00 mL min−1 (column 2 = 1.20 mL min−1) |
| Detector temperature | - |
| Makeup gas | - |
| Oven program | 70 °C for 2 min 25 °C/min to 150 °C for 0 min; 3 °C/min to 200 °C for 0 min; 8 °C/min to 280 °C for 10 min hold time |
| MS transfer line temperature | 280 °C |
| Backflush settings | 5 min during post-run / 310 °C |
| Aux EPC pressure | ~50 psi |
| Inlet pressure | ~2 psi |
| Column pressure | ~3 psi |
| Electron energy | 70 eV |
| MS1 and MS2 resolution | Wide |
| Collision cell | 1.5 mL min−1 N2 and 2.25 mL min−1 He |
| Source temperature | 300 °C |
| Quad temperatures | 150 °C |