Fig. 1.

The structure construct pWYP21404 and the integration of tobacco chloroplast genome. A Expression cassette of the foreign genes. smGFP, the soluble, modified green fluorescent protein encoding sequence; linker, the linker peptide [(GSSSS)₃-DDDDK] coding sequence; EGF, the epidermal growth factor encoding gene; aadA, the aminoglycoside adenyltransferase coding gene. Prrn, the promoter of the rrn opren of tobacco chloroplast genome; T7-g10, 5’ UTR of the T7 phage g10 gene; Trps16, the terminator of the tobacco plastid gene rps16. RBS, the ribosomal binding site of tobacco plastid rbcL gene; attP, attB, phage and bacterial recognize sites of the φ31 integrase. 16Srrn and 23Srrn, the ribosome 16 S rRNA and 23 S rRNA genes of tobacco chloroplast genome respectively; trnI and trnA, the transfer RNA of isoleucine and alanine encoding genes of tobacco chloroplast genome respectively. B The wild-type structure of flanking the target site (Nsi I) of the tobacco chloroplast. The restriction enzymes BamH I and Kpn I were used to digest the DNA for southern blotting analysis; the short, thick line indicates the probe used in DNA for southern blotting analysis. C The structure of the recombinant tobacco chloroplast genome