Fig. 3.

Southern blotting analysis and expression of GFP-EGF in tobacco plants. A Southern blotting used the probe indicated in Fig. 1, five micrograms of DNAs for each were digested by Bam HI/Kpn I and used. GFP, the GEP standard protein; wt, wild-type plant; 1–4, transplastomic plants. B, C The SDS-PAGE and the Western blotting analysis of the GFP-EGF expression. Wt, wild-type plant; 1–4, transplastomic plants. D, E The Expression level of GFP-EGF in transplastomic tobacco plants. Six tests were repeated for each line, and the data were shown as mean ± SD. Average, the average expression of the 10 tested lines; line1-line10, different transplastomic tobacco lines