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. Author manuscript; available in PMC: 2023 Dec 6.
Published in final edited form as: Cell Metab. 2022 Dec 6;34(12):1947–1959.e5. doi: 10.1016/j.cmet.2022.11.004

Figure 2. Circulating NAM enters the gut lumen and supports microbial NAD biosynthesis.

Figure 2.

A. NAM levels in the serum samples collected from portal, hepatic and tail vein 15 min after retro-orbital injection of different doses of [2,4,5,6-2H]-NAM. n=2–3 mice per group.

B. Labeled NAM detected in the gut lumen of mice treated as in A.

C. Labeled and unlabeled NA detected in the luminal samples collected from different parts of the gastrointestinal tract of mice treated as in A.

D. Labeled and unlabeled NA detected in the serum samples collected from mice treated as in A.

E. Labeled and unlabeled NAM in luminal samples collected from germ-free (GF) and conventional (Conv) mice retro-orbitally injected with 5 μmoles of [2,4,5,6-2H]-NAM and sacrificed after 2h. n=4 mice per group. (n=4 per group; Tukey multiple comparison test following two-way ANOVA, **** = p < 0.0001).

F. Labeled and unlabeled NA in the luminal samples collected from mice treated as in E. (n=4 per group; Tukey test following two-way ANOVA, **** = p < 0.0001).