Skip to main content
. 2022 Dec 8;50(22):13011–13025. doi: 10.1093/nar/gkac1140

Figure 1.

Figure 1.

TCP-seq of 80S ribosomes. (A) Cartoon depicting the main steps of the optimised TCP-seq protocol (created with BioRender.com). S. pombe cells are snap chilled before cross-linking with formaldehyde. Cells are harvested and frozen. After lysis, cell extracts are treated with RNase I and separated by density centrifugation. Fractions containing either the 40S or 80S complexes are purified and decross-linked. FP RNAs are isolated on gels for library preparation and sequencing. (B) Metagene heatmap showing the relationship between FP length and FP location around the aligned initiation sites for 80S libraries. The distance between the 5′ end of the FP and the start codon is plotted. The colours indicate the number of FPs. (C) As in (A), but the distance to the 3′ end of the FP is plotted. (D) Projection of the data in B along the x-axis. (E) Projection of the data in (B) along the x-axis.