Figure 5.

Distribution of 80S and 40S FPs of different lengths for 12 selected transcripts. Data from Figure 3 and Figure 4 segmented by FP lengths. X-axes: distance to the initiation codon; transcription start sites (black dashed lines); translation initiation sites for main coding sequence (dashed red lines); AUG-starting uORFs (dotted blue lines). Y-axes: Relative FP numbers were obtained by normalising 80S/40S FP numbers by their corresponding maximum value (all lengths): FP length ≤35 nucleotides (dark blue), FP length 36–59 (light blue), FP length ≥60 (orange). Note the whole length of the FPs is plotted (cf. Figures 1 and 2 where only 5′/3′ ends are shown). All data are for replicate 2 except for gcn5, which shows aggregated data for three replicates.