Figure 1.

Recruitment of PCH2 to the synaptonemal complex is independent of the chromosome axis and its co-factor COMET. (A) A schematic representation of the structure of synapsing chromosomes and the key proteins involved. (B) Localization of PCH2:GFP in the male meiocytes of wild-type (WT), asy1, asy3, and comet mutant plants at pachytene or pachytene-like stages using confocal laser scanning microscope. Red arrowheads indicate the short stretches of PCH2 signal localizing at synapsed chromosomal regions. Bars: 10 μm. (C) Co-immunolocalization of PCH2 and ZYP1 in WT, asy1 and comet mutants at pachytene or pachytene-like stages and at late prophase I. Bars: 5 μm.