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. 2022 Dec 12;50(22):12924–12937. doi: 10.1093/nar/gkac1160

Figure 6.

Figure 6.

ZYP1 and ZYP1-mediated ASY1 removal regulate the formation of wild-type level of COs. (A) Scatter dot plot of the estimation of minimum chiasma number in WT, zyp1a/b-1, zyp1a/b-2, zyp1a/b-3 and ZYP1BΔ661–725 (zyp1a/b-1) mutant plants. The statistical analysis was performed by one-way ANOVA with Tukey's correction for multiple comparison test. (B) Z-stack imaging of MLH1:GFP in WT, zyp1a/b-1 and ZYP1BΔ661–725 (zyp1a/b-1) mutant plants at late prophase of male meiocytes. The upper panel shows one representative z-stack image for each genotype and lower panel depicts the maximum intensity projection (MIP) of z-stack images displaying all MLH1 foci from each meiocyte. Bars: 10 μm. (C) Scatter dot plot of the number of MLH1 foci in WT, zyp1a/b-1, zyp1a/b-2, and ZYP1BΔ661–725 (zyp1a/b-1) mutant plants. The statistical analysis was performed by one-way ANOVA with Dunnett's correction for multiple comparison test. * and ** indicate significant differences at P < 0.05 and P < 0.01, respectively.