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Rates for clamp closing reactions on DNA-SSB substrates (40 nM) with different lengths ss dT 5′ overhangs
| 5′ overhang | [SSB]/[DNA] | afast | kfast (s−1) | aslow | kslow (s−1) |
|---|---|---|---|---|---|
| ss dT35 | 1.5 | 1 ± 0.03 | 8.6 ± 0.4 | 0.1 ± 0.06 | 0.1 ± 0.05 |
| ss dT65 | 1.5 | 1 ± 0.05 | 9.1 ± 0.2 | 0.1 ± 0.09 | 0.4 ± 0.4 |
| ss dT80 | 1.5 | 1 ± 0.06 | 10.2 ± 0.1 | 0.05 ± 0.01 | 1.4 ± 0.4 |
| ss dT80 | 5 | 1 ± 0.04 | 8.7 ± 0.1 | 0.1 ± 0.05 | 0.4 ± 0.2 |
*Amplitudes (a) and rates (k) with standard deviations are given for three independent measurements.
