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. 2022 Dec 13;50(22):12938–12950. doi: 10.1093/nar/gkac1164

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Synthetic adhesion enhances DNA tranference in complex triparental mating schemes. Schematic representations of the experiments, conjugation frequencies (CFs) and mobilization frequencies (MFs) (transconjugants/recipient) shown in logarithmic scale of RP4 and RSF1010 plasmids after grown 2 h. Donor 1 (D1) and recipients (R) both carried either pNVgfp (Control; C) or pNVtir1 (SAtir). Donor 2 (D2) cells were EcM1flu::TirMA plus RSF1010. D1 cells also carried RP4, which mobilizes RSF1010 upon conjugation to D2 cells. ***P < 0.001, by unpaired Student's t-test.