. 2022 Nov 23;38(1):89–102. doi: 10.1093/humrep/deac250

Table II.

Proteins with significantly differing alterations between study groups during the 9-week trial.

				Fold changes			Group comparison
Fraction	Accession	Gene	Protein name	EE + DNG	EV + DNG	DNG	A	B	C
LOW	Q9NPH3	IL1RAP	Interleukin-1 receptor accessory protein	3.0^*	1.7^*	1.2	S	S	S
LOW	Q9UGM5	FETUB	Fetuin-B	2.0^*	1.4^*	1.2	S	S	S
LOW	P01019	AGT	Angiotensinogen	2.6^*	1.7^*	1.2	S	S	S
LOW	P04278	SHBG	Sex hormone-binding globulin	3.2^*	1.4	1.2	S	S	NS
LOW	O75144	ICOSLG	ICOS ligand	2.2*	1.5*	1.1	S	S	S
LOW	A0A087X1L8	LOC102723996	Ig-like domain-containing protein	2.2*	1.5*	1.1	S	S	S
LOW	P08185	SERPINA6	Corticosteroid-binding globulin	3.2^*	1.2	1.3	S	S	NS
LOW	Q9HD23	MRS2	Magnesium transporter MRS2 homolog, mitochondrial	2.1^*	1.2	1.1	S	S	NS
LOW	P08294	SOD3	Extracellular superoxide dismutase [Cu-Zn]	0.5^*	0.8	1.0	S	S	NS
LOW	Q96PD5	PGLYRP2	N-acetylmuramoyl-L-alanine amidase	0.5^*	0.9	1.1	S	S	S
LOW	Q562R1	ACTBL2	Beta-actin-like protein 2	1.7^*	1.2	1.1	S	S	NS
LOW	Q5TCS8	AK9	Adenylate kinase 9	0.5^*	0.9	1.1	S	S	S
LOW	P04196	HRG	Histidine-rich glycoprotein	0.6^*	0.9	1.1	S	S	NS
LOW	Q8N6C8	LILRA3	Leukocyte immunoglobulin-like receptor subfamily A member 3	0.6^*	0.9	1.2	S	S	S
LOW	Q04756	HGFAC	Hepatocyte growth factor activator	0.5^*	0.8	1.0	S	S	S
LOW	P01008	SERPINC1	Antithrombin-III	0.6^*	0.9	1.1	S	S	S
LOW	P07225	PROS1	Vitamin K-dependent protein S	0.7^*	1.0	1.1	S	S	NS
HIGH	P04083	ANXA1	Annexin A1	1.5^*	1.1	1.0	S	S	NS
LOW	P05155	SERPING1	Plasma protease C1 inhibitor	1.1^*	1.5	2.0	S	S	NS
HIGH	Q9NRF9	POLE3	DNA polymerase epsilon subunit 3	1.3^*	1.0	1.0	S	S	NS
HIGH	Q9Y4X3	CCL27	C-C motif chemokine 27	1.3^*	1.0	1.0	S	S	NS
LOW	P05452	CLEC3B	Tetranectin	0.6^*	0.8	1.0	S	S	S
LOW	P07357	C8A	Complement component C8 alpha chain	0.8^*	0.9	1.1	S	S	NS
LOW	P20851	C4BPB	C4b-binding protein beta chain	5.6^*	6.1	7.0	S	S	NS
HIGH	A4FU69	EFCAB5	EF-hand calcium-binding domain-containing protein 5	0.9^*	1.0	1.1	S	S	NS
HIGH	P20742	PZP	Pregnancy zone protein	1.5^*	1.0	1.0	S	S	NS
LOW	P06396	GSN	Gelsolin	0.7*	0.9	1.1	S	S	NS
	P22460	KCNA5	Voltage-gated potassium channel HK2
	Q8N1V2	CFAP52	Cilia- and flagella-associated protein 52
	Q99607	ELF4	ETS-related transcription factor Elf-4
	Q9UH62	ARMCX3	Armadillo repeat-containing X-linked protein 3
	Q9Y2M5	KLHL20	Kelch-like protein 20
HIGH	P01009	SERPINA1	Alpha-1-antitrypsin	1.4^*	1.0	1.0	S	S	NS
LOW	A5A3E0	POTEF	POTE ankyrin domain family member F	0.6^*	0.8	1.0	S	S	S
LOW	P02747	C1QC	Complement C1q subcomponent subunit C	0.8^*	0.9	1.1	NS	S	S
LOW	Q92835	INPP5D	Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1	0.8^*	0.9	1.1	S	S	NS
HIGH	P01880	IGHD	Immunoglobulin heavy constant delta	0.8^*	1.0	1.0	S	S	NS
LOW	P04003	C4BPA	C4b-binding protein alpha chain	1.9^*	2.1	2.8	S	S	NS
LOW	P51884	LUM	Lumican	0.6^*	0.8	1.0	S	S	NS
LOW	P02749	APOH	Beta-2-glycoprotein 1	331.5^*	443.3	589.0	S	S	NS
LOW	P07737	PFN1	Profilin-1	0.7^*	0.9	1.1	S	S	NS
LOW	P09871	C1S	Complement C1s subcomponent	0.8^*	0.9	1.1	S	S	NS
LOW	P13671	C6	Complement component C6	0.8^*	0.9	1.0	S	S	NS
LOW	P02748	C9	Complement component C9	0.7^*	0.9	1.0	S	S	NS
LOW	P08697	SERPINF2	Alpha-2-antiplasmin	0.8^*	1.0	1.1	S	S	NS
LOW	P01011	SERPINA3	Alpha-1-antichymotrypsin	0.7*	0.9	1.1	NS	S	NS
LOW	Q9HCY8	S100A14	Protein S100-A14	0.7*	0.9	1.1	NS	S	NS
LOW	P06727	APOA4	Apolipoprotein A-IV	10.7^*	13.0	17.2	NS	S	S
LOW	Q16473	TNXA	Putative tenascin-XA	0.6^*	0.8	1.0	S	S	NS
LOW	Q13822	ENPP2	Ectonucleotide pyrophosphatase/phosphodiesterase family member 2	1.5	0.8^*	1.0	S	NS	S
LOW	P02745	C1QA	Complement C1q subcomponent subunit A	0.7^*	0.9	1.1	S	S	NS
LOW	P00450	CP	Ceruloplasmin	1.5*	1.1	1.2	S	S	NS
LOW	Q96L14	CEP170P1	Cep170-like protein	1.5*	1.1	1.2	S	S	NS
LOW	Q9BWP8	COLEC11	Collectin-11	0.7^*	1.0	1.1	S	S	NS
LOW	P35442	THBS2	Thrombospondin-2	0.7^*	0.9	1.0	S	S	NS
LOW	Q9NQ79	CRTAC1	Cartilage acidic protein 1	0.7^*	0.9	1.0	S	S	NS
LOW	P00736	C1R	Complement C1r subcomponent	0.8^*	0.9	1.1	S	S	NS
LOW	P02746	C1QB	Complement C1q subcomponent subunit B	0.7^*	0.9	1.1	S	S	NS
LOW	P07360	C8G	Complement component C8 gamma chain	0.8^*	1.0	1.1	S	S	NS
LOW	P02776	PF4	Platelet factor 4	1.1	0.9	1.1	S	NS	S
HIGH	P08185	SERPINA6	Corticosteroid-binding globulin	1.4^*	1.0	1.0	S	S	NS
LOW	P09172	DBH	Dopamine beta-hydroxylase	0.7^*	0.9	1.1	S	S	NS
LOW	P10643	C7	Complement component C7	0.8^*	0.9	1.1	S	S	NS
LOW	Q8WXW3	PIBF1	Progesterone-induced-blocking factor 1	0.7^*	1.0	1.0	S	S	NS
LOW	Q8N531	FBXL6	F-box/LRR-repeat protein 6	0.8^*	1.0	1.0	S	S	NS
LOW	P05019	IGF1	Insulin-like growth factor I	0.8^*	0.9	1.1	S	S	NS
LOW	P05543	SERPINA7	Thyroxine-binding globulin	1.4^*	1.1	1.1	S	S	NS
LOW	Q6EMK4	VASN	Vasorin	0.7^*	0.9	1.1	S	S	NS
LOW	Q9UBP9	GULP1	PTB domain-containing engulfment adapter protein 1	0.8	0.9	1.1	NS	S	S
LOW	Q96QU8	XPO6	Exportin-6	0.7^*	0.9	1.1	S	S	NS
HIGH	Q9H078	CLPB	Caseinolytic peptidase B protein homolog	0.6^*	0.9	1.0	NS	S	NS

Proteins with a statistically different change between the groups after the Kruskal–Wallis test and Benjamini–Hochberg method (in order of significance). The Mann–Whitney U test was used for pairwise analysis. Proteins shown in italics belong to the family of proteins with similar peptides with shared statistical analysis. The high-abundance fraction indicates that the protein co-eluted with high-abundance proteins, i.e. it had probably formed a complex with highly concentrated proteins in the serum. A fold change >1 indicates an increase in protein amount, whereas a fold change between 0 and 1 reflects reduced quantity.

Statistically significant change within the group.

A: ethinylestradiol (EE) + dienogest (DNG) versus estradiol valerate (EV) + DNG. B: EE + DNG versus DNG. C: EV + DNG versus DNG. S: Mann–Whitney U test P < 0.05; NS, non-significant.