Fig. 6.

Proposed model of ID1 activation in DMG (upper panel) and its inhibition with CBD and downstream phenotypic impact (lower panel). The proposed activation model is made up of the following sub-sections: (a) H3K27M inhibits PRC2, leading to global decreases in H3K27me3 and subsequently allowing for increased H3K27ac. (b) Regional or tissue-specific factors and/or (c) constitutively activating ACVR1 mutations increase ID1 expression via SMAD protein signaling. ID1 inhibition by CBD treatment, which partially acts through increasing intracellular levels of reactive oxygen species (ROS), regulating migration and invasion in an ID1-dependent manner, while inhibiting proliferation in an ID1-independent manner. Image created with BioRender.