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. 2023 Jan 7;13:361. doi: 10.1038/s41598-023-27593-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of WLZYW on DNP-IgE/BSA-induced RBL-2H3 cells. CCK8 assay indicated that WLZYW was not cytotoxic to RBL-2H3 cells in certain range (A). QRT-PCR showed that WLZYW reduced mRNA expression of TNF-α (B) and VEGFa (C). Elisa showed that WLZYW decreased VEGFa protein expression (E), but only TNF-α protein expression decreased at high concentrations (D). Values represent mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns means no significant difference.