Key Resources Table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Ubiquitin (clone Ubi-1)	MilliporeSigma	Cat#MAB1510-I
Anti-Mouse IgG, HRP conjugate	MilliporeSigma	Cat#12–349
Anti-Legionella	Scrum	Cat#17457
Anti-Legionella	BioAcademia	Cat#64–100
Anti-HA	MBL	Cat#561
Anti-VCP	Abcam	Cat#ab11433
Anti-Rabbit IgG, Alexa488 conjugate	Invitrogen	Cat#A11034
Anti-Rat IgG, Alexa 568 conjugate	Invitrogen	Cat#A11077
Anti-Mouse IgG, Alexa488 conjugate	Invitrogen	Cat#A11029
Anti-Rabbit IgG, RhodamineRedX conjugate	Invitrogen	Cat#R6394
Bacterial strains
See Table S1 for a full list of bacterial strains
Chemicals and recombinant proteins
ATTO488-maleimide	ATTO-TEC	Cat#AD488
K27 diUb	UbiQ	Cat#UbiQ-015
K11 tetraUb	R&D Systems	Cat#UC-45–025
Deposited data
Crystal structure of LotAN	This study	PDB: 7UYG
Crystal structure of LotAN bound to K6 diUb	This study	PDB: 7UYH
Experimental models: Cell lines
Human: HeLa-FcγRII	Laboratory of K. Arasaki	Ref59
Mouse: Bone marrow-derived macrophages	A/J mice	Ref60
Oligonucleotides
See Table S1 for a full list of oligonucleotides
Recombinant DNA
See Table S1 for a full list of recombinant DNA
Software and algorithms
XDS	Ref61	Version Jan 31, 2020
CCP4i2	Ref62	Version 7.1.004
Aimless	Ref63	Version 0.7.4
SHELXC/D/E	Ref64
ARP/wARP	Ref65	Version 8.0
Phaser	Ref66	Version 2.8.3
PHENIX	Ref67	Version 1.17.1
Coot	Ref68	Version 0.9
Pymol	www.pymol.org	Version 2.2.2
Prism	GraphPad	Version 9