Skip to main content
. 2023 Jan 8;13:389. doi: 10.1038/s41598-022-27348-8

Figure 2.

Figure 2

PEPMatch-predicted epitope of myelin proteolipid protein (PLP) has high similarity to experimentally validated epitopes and is in a region associated with strong T cell responses in MS patients. (A) PEPMatch-predicted peptides sharing homology with nucleocapsid were assessed for overlap with known, experimentally validated epitopes on the Immune Epitope Databases (IEDB). All known epitopes for each protein were run in a BLASTp query against the peptide(s) returned from PEPMatch. The number of homology “hits” were quantified and divided by the number of input PEPMatch peptides for that protein for normalization. (B) The PEPMatch-predicted epitope of PLP matching nucleocapsid (blue) was aligned with the full-length sequence of PLP (black) and the nucleocapsid peptide to which it matches (green) alongside examples of known epitopes of PLP identified in the literature and available on the IEDB (berry). (C) Predicted PLP structure was highlighted with known immunodominant areas previously identified as eliciting strong T cell responses preferentially in MS patients20 (cyan) against the PEPMatch-predicted epitope sharing homology with nucleocapsid (dark blue).