TABLE 2.
Ag85A-specific Ab production in B6 mice vaccinated with DNA and boosted with purified protein
| Vaccine used | Concn (ng/ml) of total Iga | IgG1 (arbitrary units/ml)b | IgG2a (arbitrary units/ml)b |
|---|---|---|---|
| MPL-A | 2.34 ± 0.08 | 1 ± 0.1 | 1 ± 0.1 |
| 85A protein | 3.70 ± 0.16 | 404 ± 17 | 1 ± 0.1 |
| 85A DNA | 4.40 ± 0.13 | 44 ± 1.26 | 151 ± 38 |
| DNA-protein | 4.71 ± 0.26 | 72 ± 4 | 350 ± 61 |
a
Ag85A-specific IGκ concentration (mean ± SD of five sera tested individually) expressed in log10 values.
b
Antibody isotype titers (determined on pooled sera) compared to a standard serum with an assigned titer of 1,000 for both isotypes.