TABLE 3.
Bacterial replication in lungs from B6 mice vaccinated with Ag85A protein, Ag85A DNA, or a DNA prime-protein boost regimen and challenged with M. tuberculosis H37Rv 2 months after the last immunization
| Vaccine used | Level of replication (no. of CFU/lung) after i.v. M. tuberculosis challengea (no. of mice)
|
||
|---|---|---|---|
| 30 days | 60 days | 90 days | |
| MPL-A-control DNA | 5.27 ± 0.20 (10) | 5.06 ± 0.23 (8) | 5.20 ± 0.22 (8) |
| BCG | 3.70 ± 0.22b (3) | NDc | 4.22 ± 0.11b (3) |
| Ag85A protein | 5.15 ± 0.26 (5) | 4.87 ± 0.13 (5) | 4.93 ± 0.20 (4) |
| Ag85A DNA | 4.82 ± 0.27b (5) | 4.93 ± 0.26 (5) | 5.18 ± 0.22 (4) |
| DNA-protein | 4.54 ± 0.16b (5) | 4.56 ± 0.25b (5) | 5.06 ± 0.25 (3) |
a
Mean ± SD (log10 values) is given for numbers of CFU/lung.
b
P < 0.005.
c
ND, not done.