TABLE 4.
Bacterial replication in lungs from B6 mice vaccinated with Ag85B DNA, boosted with recombinant Ag85B protein in SBAS2A adjuvant, and challenged with bioluminescent M. tuberculosis H37Rv
| Vaccine used | No. of CFU/lunga (no. of mice) | No. of RLU/lungb (no. of mice) |
|---|---|---|
| Control DNA | 5.09 ± 0.21 (6) | 4.02 ± 0.28 (6) |
| BCG | 3.98 ± 0.39 (5) | 3.15 ± 0.24 (5) |
| Ag85B DNA | 4.50 ± 0.14 (4) | 3.43 ± 0.20 (4) |
| DNA-protein | 3.94 ± 0.26 (5) | 3.13 ± 0.11 (5) |
a
Mean number of CFU ± SD (log10 values) as determined by plating on Middlebrook 7H11 agar.
b
Mean number of relative light units (RLU) ± SD (log10 values) as determined in a Turner Design luminometer (15-s integration time).