Table 3.
Alignment of gRNA library with Cricetulus griseus and Chinese hamster ovary (CHO) cell genome assemblies
| CHOZN v2.3 | CriGri_1.0 (2011) | C‐griseus_v1.0 (2013) | PICRH (2020) | |
|---|---|---|---|---|
| Total genes targeted (% orthologs targeted) | 18,955 (100) | 15,168 (97) | 14,976 (98) | 15,800 (98) |
| Genes with 0 bp gRNA mismatch (% of genes) | 18,955 (100) | 11,782 (78.0) | 11,801 (78.8) | 12,550 (79.4) |
| Genes with 1 bp gRNA mismatch (% of genes) | 0 (0) | 423 (3.0) | 408 (2.7) | 520 (3.3) |
| Genes with 2 bp gRNA mismatch (% of genes) | 0 (0) | 623 (4.0) | 582 (3.9) | 656 (4.2) |
| Genes with 3 bp gRNA mismatch (% of genes) | 0 (0) | 2,340 (15.0) | 2,185 (14.6) | 2,074 (13.1%) |
Note: Percentage of total genes targeted is based on the total number of orthologous genes for each specific genome as presented in Table 2. The percentage of genes with gRNAs targeting with 0, 1, 2, or 3 bp mismatches is calculated based on the number of total genes targeted.