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. 2022 Sep 7;1517(1):125–142. doi: 10.1111/nyas.14884

FIGURE 5.

FIGURE 5

Sagittal view of the adult P. discolor bat brain via histology. (A) Schematic representation indicates key anatomical structures used for protein expression analysis. The main anatomical structures are written in black, while subregions marked out by protein expression are in gray. (B) Nissl stain of the sagittal slice. Immunohistochemical detection of protein expression of (C) FoxP1, (D) FoxP2, (E) parvalbumin, and (F) GluR1. Foxp2 N16 antibody produces high levels of fiber staining that can be seen in panel D in the corpus collosum and in the white matter of the cerebellum and brainstem. For a clearer representation of the staining, the image brightness of all the stained images was altered using a linear adjustment of the mid‐tones of the levels parameter in Photoshop (mid‐tones of Image of Nissl stains were adjusted to 0.5, and images of protein expression were adjusted to 0.3). The scale bar indicates 500 μm. Abbreviations: Acb, nucleus accumbens; cc, corpus callosum; Cd, caudate nucleus; Fr, frontal cortex; Hip, hippocampus; IC, inferior colliculus; Occ, occipital cortex; Md, medulla; Par, parietal cortex; RT, reticular thalamic nucleus; SC, superior colliculus; Temp, temporal cortex; VPL, ventral posterolateral thalamic nucleus; VPM, ventral posteromedial thalamic nucleus.