FIG. 5.

MS-Fit search of peptide monoisotopic mass spectra identified MOMP of C. pneumoniae. Proteins obtained by immunoprecipitation of purified C. pneumoniae EBs with the GZD1E8 (A) and RR-402 (B) MAbs were processed by MS. Masses indicated that fragments 1 to 11 (A) and 1 to 8 (B) matched known MOMP sequence fragments. Trypsin (T) autodigestion peptides were used for internal calibration of the spectrum prior to peptide mass assignment for MS-Fit searching of the NCBI database. The MOMP sequence aligned with the matching peptide masses identified with MAbs GZD1E8 (C) and RR-402 (D).