FIGURE 7.

Programmed death ligand 1 (PD‐L1) binds to the 1–86aa fragment of KRAS. (A). Confocal picture showing diamidine‐phenylindole (DAPI)‐stained nuclei (blue), PD‐L1 (green), K‐RAS (red) or a merged picture. The scale bar in 400× images indicates 50 μm. (B). Co‐IP showed that the binding of PD‐L1 to K‐RAS could be detected, excluding the nonspecific binding of rabbit IgG to PD‐L1. Anti‐KRAS (produced in rabbits) immunobound with the complex and anti‐K‐RAS (produced in rabbits) and anti‐PD‐L1 (produced in mice) antibodies were used for detection. (C). Spearman's correlation analysis of KRAS expression and PD‐L1 expression performed in the TCGA dataset. (D). Schematic diagram of K‐RAS truncates with flag tags. (E). Coimmunoprecipitation showed that PD‐L1 binds to the 1–86 aa domain of KRAS. All experiments were repeated at least three times independently