Figure 2.

Chemoenzymatic synthesis of N‐terminally modified microviridin J and microviridin B derivatives by using LP‐MvdD alone (blue line) or in combination with LP‐MvdC (green line). a)–f) HPLC monitoring (absorbance at 199 nm) of enzyme assays. Novel peaks correspond to monocyclic (CP_cy1), bicyclic (CP_cy2) and tricyclic (CP_cy3) microviridin products as indicated by mass spectrometric analysis (Figures S2–S29, Table S1). All tricyclic microviridins (1–6) were characterized and confirmed by tandem ESI‐MS/MS experiments (Figures S30–S35).