Figure 2.

Chemo‐cat NIR retains chemotactic activity in mouse macrophages. A) Schematic illustration of transwell assays in RAW264.7 macrophages where migrated cells were stained with Hoechst 33342 after 2 h incubation with media containing increasing concentrations of unlabeled mCCL2 or chemo‐cat NIR (0, 10, 30 and 100 ng mL⁻¹). B) Representative fluorescence microscopy images (from n=3) of Hoechst‐stained migrated macrophages after incubation with mCCL2 or chemo‐cat NIR. Scale bar: 10 μm. Cell counting was performed using ImageJ and values are presented as means±SD (n=3). P values were determined using one‐way ANOVA (n.s. for p>0.05).