Figure 3.

Chemo‐cat NIR enters macrophages in a CCR2‐dependent manner and is cleaved by intracellular cathepsins. A) RAW264.7 macrophages were pre‐incubated or not with the inhibitors (RS504393 or FJD005, both 2.5 μM for 5 min) followed by labeling with chemo‐cat NIR or compound 6 (250 nM) at 37 °C. Cells were lysed and proteins were separated by SDS‐PAGE and visualized by in‐gel fluorescence scanning. B,C) Representative fluorescence and brightfield microscopy images (from n=3) of RAW264.7 macrophages after incubation with chemo‐cat NIR or compound 6 (red, 250 nM) with or without treatment of RS504393 (B) and co‐staining with anti‐LAMP1 (green) and DAPI (blue) (C). Scale bars: 10 μm.