Fig. 3.

Teriflunomide administration positively affects remyelination-related oligodendroglial dynamics. A–E″ Representative images of oligodendroglial differentiation-associated protein markers in the caudal corpus callosum after 1 week of remyelination. A, A″, F) Degree of remyelination was determined as the percentage of PLP-positive area in the defined region of interest between CPZ-vehicle treated, CPZ with teriflunomide constant treatment (CPZ-Teri constant) and CPZ with teriflunomide pulse treatment (CPZ-Teri pulse) groups. The impact on oligodendroglial cell differentiation and maturation was assessed by the number/mm² of Sox10-positive cells and the relative percentage of double positive cells (Sox10 + /CC1 + ; B, C″,G, H), (Sox10 + /Mash1 + ; D, E″, I) along the same area of the corpus callosum. Number of animals per analysis n = 6. Data are shown as mean values (horizontal lines), while error bars represent standard error of the mean (SEM; vertical lines). Plots also show all individual data points. Statistical significance was calculated using Kruskal–Wallis test with Dunn’s post-test (F, G, H) and Tukey’s range test following one-way ANOVA (I). Data were considered statistically significant (95% confidence interval) at *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars: 50 µm, 20 µm