FIG. 3.

RPA analysis for expression of bovine NRAMP1-R in stable RAW264.7 transfectants. Lane 3, parental RAW264.7 cells; lanes 4 through 11, stable bovine NRAMP1-R transfected clones. A protected fragment of the expected size is observed for the clones in lanes 4, 5, 7, 8, and 9; no signal is obtained with the parental cells (lane 3). The presence of a larger protected fragment can be attributed to the presence of secondary structure in the antisense probe. The lower dominant band is β-actin. The respective sizes of the protected fragments are 289 (NRAMP1-R) and 250 (β-actin; positive control) bases. Full-length antisense probes for β-actin and NRAMP1-R are shown in lanes 1 and 2. Exposure is 3 h.