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. Author manuscript; available in PMC: 2023 Jan 8.
Published in final edited form as: Exp Eye Res. 2022 Sep 2;224:109213. doi: 10.1016/j.exer.2022.109213

Fig. 9: Treatment with anti-CCL3 improved retention of retinal function and reduced intraocular inflammation.

Fig. 9:

Treatment with anti-CCL3 antibody alone, with or without gatifloxacin (GAT) arrested intraocular inflammation and retained retinal function. C57BL/6J mice were intravitreally injected with 100 CFU B. cereus. At 2 hours postinfection, groups of infected eyes were treated with 250 ng/0.5μl anti-CCL3, 1.25ug/0.5μl GAT, 0.5μl PBS containing 250 ng Anti-CCL3 and 1.25μg GAT, and 0.5μg/0.5μl nonspecific isotype IgG2A (Isotype). All eyes were analyzed at 10 hours postinfection. (9A) Treatment with gatifloxacin sterilized the eye in both GAT alone and GAT+anti-CCL3 treated eyes. No differences in bacterial counts were observed between untreated and anti-CCL3 (P=0.3282) or isotype-treated (P=0.5358) eyes. (9B) Compared to untreated eyes A-wave function was significantly greater in GAT alone (P=0.026) and GAT+anti-CCL3 (P=0.0225) treated eyes, but not in anti-CCL3 alone (P=0.1807) treated eyes. (8C) Compared to untreated eyes, B-wave function was significantly retained in GAT alone (P=0.0043) and GAT+anti-CCL3 (P=0.0225) treated eyes but not in anti-CCL3 alone (P=0.2949) treated eyes. No differences in A- or B-wave retention was detected between untreated and isotype-treated eyes. (9D) MPO concentrations in infected eyes of mice treated with anti-CCL3 antibody with (P=0.0079) or without antibiotics (P=0.0079) and antibiotics alone (P=0.0159) were significantly less than in untreated eyes. Values represent means ± SEM for n ≥ 5 eyes per group per time point with at least 2 independent experiments. *P ≤ 0.05, **P≤0.01, and nsP≥0.05. (9E) Histology analysis of H&E staining showed stark contrast between uninfected and untreated or isotype-treated mice. Compared to uninfected, untreated or isotype-treated mice eyes were inflamed, with the loss of partial retinal architecture. In contrast, there was mild inflammation and undamaged retinal layers in infected eyes treated with GAT alone and GAT+anti-CCL3. Retinal layers in anti-CCL3 alone treated eyes were intact but eyes were highly inflamed. Original magnification, ×10. Sections are representative of two eyes in each group.